Development and Application of a Validated Liquid Chromatography-Mass Spectrometry Method for the Determination of Dexchlorpheniramine Maleate in Human Plasma

نویسندگان

  • Gopinath Ram CADRAT, JSS College of Pharmacy
  • Mahesh Kumar Siddaiah TIFAC CORE, JSS College of Pharmacy
  • Nanjan Joghee TIFAC CORE, JSS College of Pharmacy
  • Rajan Sekar CADRAT, JSS College of Pharmacy
  • Suresh Bhojraj Principal, JSS College of Pharmacy, Rock lands, Ootacamund, India
چکیده مقاله:

     A convenient liquid chromatographic-single Quadrupole mass spectrometric (LC-MS) method was developed and validated for dexchlorpheniramine maleate (INN name: chlorphenamine) determination in human plasma. The need for just a single liquid-liquid extraction with ethyl acetate and being highly sensitive were the advantages of this method. The linearity was also excellent over the range of 1 to 150 ng.ml-1 of dexchlorpheniramine maleate concentration. The method was statistically validated for its selectivity, linearity, precision and robustness. This method was successfully applied to the analysis of chlorpheniramine maleate in clinical studies.

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development and application of a validated liquid chromatography-mass spectrometry method for the determination of dexchlorpheniramine maleate in human plasma

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عنوان ژورنال

دوره 3  شماره 3

صفحات  161- 170

تاریخ انتشار 2007-07-01

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